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pdonr223 egfr wt  (Addgene inc)


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    Addgene inc pdonr223 egfr wt
    Pdonr223 Egfr Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdonr223+egfr+wt/pDONR223_EGFR_WT+(Plasmid+%2381926)/pmc09248364-38-0-2
    Average 93 stars, based on 1 article reviews
    pdonr223 egfr wt - by Bioz Stars, 2026-10
    93/100 stars

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    Clone Assay:

    Article Title: Time-resolved proximity labeling of protein networks associated with ligand-activated EGFR
    Article Snippet: .. Gateway entry clones pDONR223-EGFR-WT (#81926) were purchased from Addgene. pLEX-305 (#41390) lentiviral backbone vector was engineered to substitute the hPKG (phosphoglycerate kinase) promoter with the CMV (human cytomegalovirus) promoter plus APEX2 tag in the C-terminus of the expression gene. pDONR223_EGFR_WT (#81926) were transferred to pLEX305_Cterm_APEX2 destination vector using Gateway LR Clonase II enzyme mix (Thermo Fisher) based on the provided protocol. .. Gateway cloning product was transformed into One ShotTM BL21(DE3) chemically competent E.coli (Thermo Fisher).

    Article Title: Time-resolved proximity labeling of protein networks associated with ligand-activated EGFR
    Article Snippet: .. Gateway entry clones pDONR223-EGFR-WT (#81926) were purchased from Addgene. pLEX-305 (#41390) lentiviral backbone vector was engineered to substitute the hPKG (phosphoglycerate kinase) promoter with the CMV (human cytomegalovirus) promoter plus APEX2 tag in the C-terminus of the expression gene. pDONR223_EGFR_WT (#81926) were transferred to pLEX305_Cterm_APEX2 destination vector using Gateway LR Clonase II enzyme mix (Thermo Fisher) based on the provided protocol. .. Gateway cloning product was transformed into One ShotTM BL21(DE3) chemically competent E.coli (Thermo Fisher).

    Expressing:

    Article Title: Time-resolved proximity labeling of protein networks associated with ligand-activated EGFR
    Article Snippet: .. Gateway entry clones pDONR223-EGFR-WT (#81926) were purchased from Addgene. pLEX-305 (#41390) lentiviral backbone vector was engineered to substitute the hPKG (phosphoglycerate kinase) promoter with the CMV (human cytomegalovirus) promoter plus APEX2 tag in the C-terminus of the expression gene. pDONR223_EGFR_WT (#81926) were transferred to pLEX305_Cterm_APEX2 destination vector using Gateway LR Clonase II enzyme mix (Thermo Fisher) based on the provided protocol. .. Gateway cloning product was transformed into One ShotTM BL21(DE3) chemically competent E.coli (Thermo Fisher).

    Article Title: Time-resolved proximity labeling of protein networks associated with ligand-activated EGFR
    Article Snippet: .. Gateway entry clones pDONR223-EGFR-WT (#81926) were purchased from Addgene. pLEX-305 (#41390) lentiviral backbone vector was engineered to substitute the hPKG (phosphoglycerate kinase) promoter with the CMV (human cytomegalovirus) promoter plus APEX2 tag in the C-terminus of the expression gene. pDONR223_EGFR_WT (#81926) were transferred to pLEX305_Cterm_APEX2 destination vector using Gateway LR Clonase II enzyme mix (Thermo Fisher) based on the provided protocol. .. Gateway cloning product was transformed into One ShotTM BL21(DE3) chemically competent E.coli (Thermo Fisher).



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    pdonr223 egfr wt  (Addgene inc)
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    gateway entry clones pdonr223 egfr wt  (Addgene inc)
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    Journal: Cell reports

    Article Title: Time-resolved proximity labeling of protein networks associated with ligand-activated EGFR

    doi: 10.1016/j.celrep.2022.110950

    Figure Lengend Snippet:

    Article Snippet: Gateway entry clones pDONR223-EGFR-WT (#81926) were purchased from Addgene. pLEX-305 (#41390) lentiviral backbone vector was engineered to substitute the hPKG (phosphoglycerate kinase) promoter with the CMV (human cytomegalovirus) promoter plus APEX2 tag in the C-terminus of the expression gene. pDONR223_EGFR_WT (#81926) were transferred to pLEX305_Cterm_APEX2 destination vector using Gateway LR Clonase II enzyme mix (Thermo Fisher) based on the provided protocol.

    Techniques: Transduction, Recombinant, Mass Spectrometry, Sequencing, Software